Holders Paris Saint-Germain are through to the Coupe de France quarter-finals, but were taken to extra-time by amateur side Villefranche-sur-Saône, while Jordan Siebatcheu was Stade Rennais FC's matchwinner against LOSC.
FC Nantes downed Toulouse 2-0, goals from Kalifa Coulibaly and Anthony Limbombe ensuring progression along with Dijon, who beat D4 side Croix 3-0, and SM Caen, who trumped SC Bastia 5-3 on penalties (2-2).
Amiens SC boss Christophe Pélissier admitted his side have a lot of improving to do before Sunday, when they will run back Thursday's 2-0 Coupe de France loss at home to Lyon in Ligue 1 Conforama's Week 22.
Lyon booked their place in the last 16 with a solid display away to Amiens, Moussa Dembélé and Léo Dubois striking in the first half to clinch a 2-0 win despite losing defender Marçal to a red card just before the break.
PSG have announced that superstar Neymar hobbled off in their 2-0 Coupe de France last 32 win over Strasbourg with a recurrence of a foot injury that could see him miss the capital club's crunch clash with Manchester United.
Paris Saint-Germain boss Thomas Tuchel admitted there were concerns over Neymar's injury in the 2-0 win over Strasbourg to reach the last 16; ASSE boss Jean-Louis Gasset was horrified by his side's capitulation to Dijon.